Bagchi M, Emanuel K
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, Michigan.
Lens Eye Toxic Res. 1991;8(1):61-74.
Energy dispersive x-ray microprobe analysis was used to determine the distribution of chloride, potassium, phosphorus and sulfur in the epithelial cells of the lenses obtained from 3 to 7 month old Emory mice and 7 month old cataract resistant strain of Emory mice. Rapidly frozen lenses were fractured in the frozen state and lyophilized. The anterior epithelial cells were analyzed from equator to equator. The results show that the epithelial cells of the 7 month old Emory mouse lens have considerably higher amounts of chloride, sulfur, potassium and phosphorus. Presence of increased amount of potassium in the epithelial cells is intriguing. The data obtained from these experiments show that the changes in the elemental levels of epithelial cells are similar to observed alteration found in the lens fiber mass of 7 month old Emory mouse.
能量色散X射线微探针分析用于确定从3至7月龄的埃默里小鼠以及7月龄抗白内障品系的埃默里小鼠获取的晶状体上皮细胞中氯、钾、磷和硫的分布。快速冷冻的晶状体在冷冻状态下断裂并冻干。从赤道到赤道对前上皮细胞进行分析。结果表明，7月龄埃默里小鼠晶状体的上皮细胞中氯、硫、钾和磷的含量明显更高。上皮细胞中钾含量增加很有意思。从这些实验获得的数据表明，上皮细胞元素水平的变化与在7月龄埃默里小鼠晶状体纤维团中观察到的变化相似。
