Kremser K, Schön H J, Lohninger A, Prager C M, Kramar R, Böck P
Institut für Medizinische Chemie, Universität Wien, Osterreich.
Eur J Clin Chem Clin Biochem. 1991 Feb;29(2):151-8.
Adult male rats were fed a standard diet containing 25 mg/kg L-thyroxine for 2 weeks. The hyperthyreotic condition of the animals was checked by monitoring the metabolic rates and liver glycerol-3-phosphate dehydrogenase. In the postnuclear fraction of the lung the activity of fatty acyl-CoA oxidase, the enzyme responsible for the rate limiting first step of peroxisomal fatty acid beta-oxidation, showed a twofold increase. Catalase, the marker enzyme of peroxisomes, showed a similar increase. Electron microscopic examination of alveolar type II cells did not reveal changes in the number and distribution frequency of peroxisomes and lamellar bodies. Similarly the content of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (dipalmitoyl phosphatidylcholine), the main constituent of alveolar surfactant, was not altered significantly by thyroxine feeding. On the other hand the volume density of the peroxisomal compartment was found to be doubled according to the measured increase of catalase and acyl-CoA oxidase. Our data suggest that the induction of peroxisomal matrix enzymes, such as catalase and fatty acyl-CoA oxidase, does not influence the surfactant content.
成年雄性大鼠被喂食含25毫克/千克L-甲状腺素的标准饮食2周。通过监测代谢率和肝脏甘油-3-磷酸脱氢酶来检查动物的甲状腺功能亢进状态。在肺的核后部分，负责过氧化物酶体脂肪酸β氧化限速第一步的酶——脂肪酰辅酶A氧化酶的活性增加了两倍。过氧化物酶体的标志酶过氧化氢酶也有类似增加。对II型肺泡细胞的电子显微镜检查未发现过氧化物酶体和板层小体的数量及分布频率有变化。同样，甲状腺素喂养并未显著改变肺泡表面活性物质的主要成分1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（二棕榈酰磷脂酰胆碱）的含量。另一方面，根据测得的过氧化氢酶和酰基辅酶A氧化酶的增加，发现过氧化物酶体区室的体积密度增加了一倍。我们的数据表明，过氧化氢酶和脂肪酰辅酶A氧化酶等过氧化物酶体基质酶的诱导不会影响表面活性物质的含量。
