SR140333 可拮抗 NK-1 介导的人乳腺癌细胞系 T47D 的增殖。
SR140333 counteracts NK-1 mediated cell proliferation in human breast cancer cell line T47D.
机构信息
Department of Pathology, Qingdao Municipal Hospital, 5 Donghai Middle Road, Qingdao, Shandong, 266071, China.
出版信息
J Exp Clin Cancer Res. 2010 May 24;29(1):55. doi: 10.1186/1756-9966-29-55.
BACKGROUND
It has been demonstrated that certain NK-1 antagonists could reduce proliferation of several cancer cell lines, however, it is unknown whether SR140333 exerts proliferation inhibition in breast cancer cell line.
METHODS
Immunohistochemical staining was carried out to investigate the immunolocation of NK-1 in breast cancer tissues and T47D cell line, thereafter, various concentrations of [Sar9, Met(O2)11]substance P and SR140333 were applied alone or combined. MTT assay was applied to detect cytoactivation and coulter counter was to detect growth curve. The Hoechst33258 staining was performed to detect apoptosis.
RESULTS
We found that breast cancer and T47D cells bear positive expression of NK-1. SR140333 inhibited cell growth in a dose dependent manner. Furthermore, SR140333 could counteract [Sar9, Met(O2)11]substance P induced proliferation. Hoechst33258 staining revealed the presence of apoptosis after SR140333 treatment.
CONCLUSIONS
Our study demonstrated SR140333 exert proliferation inhibition in breast cancer cell line T47D and indicates NK-1 play a central role in the substance P related cell proliferation in breast cancer.
背景
已经证实某些 NK-1 拮抗剂能够抑制多种癌细胞系的增殖，然而，目前尚不清楚 SR140333 是否对乳腺癌细胞系具有增殖抑制作用。
方法
通过免疫组织化学染色来研究 NK-1 在乳腺癌组织和 T47D 细胞系中的免疫定位，然后单独或联合应用不同浓度的[Sar9，Met（O2）11]物质 P 和 SR140333。MTT 法检测细胞激活，库尔特计数器检测生长曲线。采用 Hoechst33258 染色法检测细胞凋亡。
结果
我们发现乳腺癌和 T47D 细胞呈 NK-1 阳性表达。SR140333 呈剂量依赖性抑制细胞生长。此外，SR140333 可拮抗 [Sar9，Met（O2）11]物质 P 诱导的增殖。Hoechst33258 染色显示 SR140333 处理后存在细胞凋亡。
结论
本研究表明 SR140333 对乳腺癌细胞系 T47D 具有增殖抑制作用，并表明 NK-1 在乳腺癌中物质 P 相关细胞增殖中起核心作用。
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