James Cook University Hospital, Middlesbrough, UK.
Angiology. 2010 Jul;61(5):437-42. doi: 10.1177/0003319710366128. Epub 2010 May 24.
We assessed the effect of novel immunotherapeutic heat-killed bacterial (Actinomycetales) preparations on the development of myointimal hyperplasia (MIH) in a rat carotid balloon trauma model and the effect on the immune response by measuring the expression of interferon gamma (IFN-gamma; (Th1) and interleukin 4 (IL-4; Th2). There was a significant reduction (P < .001) in intima/media ratios (mean +/- SEM) in the rats treated by immunomodulation (0.52 +/- 0.03 Gordonia bronchialis, 0.60 +/- 0.03 Rhodococcus coprophilus, 0.43 +/- 0.03 Tsukamurella inchonensis, 0.37 +/- 0.03 Mycobacterium vaccae), in comparison with untreated controls (0.91 +/- 0.05). Postballoon trauma G bronchialis increased messenger RNA (mRNA) IFN-gamma (P < .02) and reduced mRNA IL-4 (P < .05). R coprophilus, T inchonensis, and M vaccae significantly increased production of mRNA IFN-gamma (P < .001). R coprophilus and M vaccae also decreased production of mRNA IL-4 (P < .05, P < .01). Treatment with heat-killed Actinomycetales inhibits MIH through a combination of enhanced Th1 and attenuated Th2 response. Immunomodulation may provide a novel therapeutic option to prevent restenosis.
我们评估了新型免疫治疗热灭活细菌（放线菌目）制剂在大鼠颈动脉球囊损伤模型中对肌内膜增生（MIH）发展的影响，并通过测量干扰素 γ（IFN-γ；（Th1）和白细胞介素 4（IL-4；Th2）的表达来评估其对免疫反应的影响。经免疫调节治疗的大鼠内膜/中膜比值（平均值 +/- SEM）显著降低（P <.001）（0.52 +/- 0.03 戈登氏菌，0.60 +/- 0.03 粪球菌，0.43 +/- 0.03 居泉菌，0.37 +/- 0.03 牛型分枝杆菌），与未治疗对照组（0.91 +/- 0.05）相比。球囊损伤后 G 支气管增加信使 RNA（mRNA）IFN-γ（P <.02）并减少 mRNA IL-4（P <.05）。R 粪球菌、T 居泉菌和 M 牛型分枝杆菌显著增加 mRNA IFN-γ 的产生（P <.001）。R 粪球菌和 M 牛型分枝杆菌也减少了 mRNA IL-4 的产生（P <.05，P <.01）。热灭活放线菌治疗通过增强 Th1 和减弱 Th2 反应的组合抑制 MIH。免疫调节可能为预防再狭窄提供一种新的治疗选择。
