高压一氧化二氮和氨甲蝶呤抑制四种癌细胞生长。

Growth suppression of four cancer cells by hyperbaric nitrous oxide and methotrexate.

机构信息

Department of Anesthesiology and Pain Medicine, Seoul National University Hospital, Seoul, Korea.

出版信息

Korean J Anesthesiol. 2010 Jan;58(1):61-9. doi: 10.4097/kjae.2010.58.1.61. Epub 2010 Jan 31.

BACKGROUND

Nitrous oxide concentration is easily controlled by respiratory ventilation. It suppresses bone marrow via the inhibition of thymidylate synthesis. The aim of this work was to determine the optimal pressure and exposure duration of nitrous oxide, as well as methotrexate concentration that maximizes the suppression of 4 cancer cells: CCRF-CEM, K562, A549 and MDA-MB-231.

METHODS

Each cancer cell was cultured in a hyperbaric chamber at 1, 2 and 3 atmosphere of 74% nitrous oxide for 24, 48, and 72 hours at 0, 0.3, 0.7, 1, 2, 5 and 10 microM methotrexate (MTX), respectively. The results were expressed in the ratio of the number of cancer cells cultured under specific conditions (S cells) to that under normal conditions (N cells).

RESULTS

The S/N ratio of CCRF-CEM cells was 87.4% in 24-hour culture, 95.0% in 48-hour culture and 115.9% in 72-hour culture (P < 0.05). The S/N ratio of K562 cells was 103.6% at 1 atm, 102.4% at 2 atm and 115.6% at 3 atm (P < 0.05). The S/N ratio of A549 cells was 94.3% at 1 atm, 94.1% at 2 atm, 99.3% at 3 atm, 96.2% in 24-hour culture, 99.2% in 48-hour culture and 99.3% in 72-hour culture (P > 0.05). However, the S/N ratio of MDA-MB 231 cells was 66.9% in 24-hour culture, 83.1% in 48 hour culture and 87.8% in 72-hour culture (P < 0.05).

CONCLUSIONS

Only the growth of the MDA-MB-231 cells was significantly reduced after a longer exposure time to nitrous oxide, but those of the other cells were not.

背景

氧化亚氮浓度可通过呼吸通气轻松控制。它通过抑制胸苷酸合成来抑制骨髓。本工作的目的是确定氧化亚氮的最佳压力和暴露时间，以及甲氨蝶呤浓度，以最大限度地抑制 4 种癌细胞：CCRF-CEM、K562、A549 和 MDA-MB-231。

方法

将每种癌细胞分别在高压舱中于 1、2 和 3 个大气压下，于 74%氧化亚氮中培养 24、48 和 72 小时，分别使用 0、0.3、0.7、1、2、5 和 10 μM 甲氨蝶呤（MTX）。结果以特定条件下培养的癌细胞数（S 细胞）与正常条件下培养的癌细胞数（N 细胞）的比值表示。

结果

CCRF-CEM 细胞的 S/N 比值在 24 小时培养时为 87.4%，在 48 小时培养时为 95.0%，在 72 小时培养时为 115.9%（P＜0.05）。K562 细胞的 S/N 比值在 1 个大气压时为 103.6%，在 2 个大气压时为 102.4%，在 3 个大气压时为 115.6%（P＜0.05）。A549 细胞的 S/N 比值在 1 个大气压时为 94.3%，在 2 个大气压时为 94.1%，在 3 个大气压时为 99.3%，在 24 小时培养时为 96.2%，在 48 小时培养时为 99.2%，在 72 小时培养时为 99.3%（P＞0.05）。然而，MDA-MB-231 细胞的 S/N 比值在 24 小时培养时为 66.9%，在 48 小时培养时为 83.1%，在 72 小时培养时为 87.8%（P＜0.05）。