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转基因内生嗜中甲基杆菌在水稻和草地贪夜蛾幼虫中的定殖 (草地贪夜蛾J.E. 史密斯,鳞翅目:夜蛾科)

Colonization of rice and Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae by genetically modified endophytic Methylobacterium mesophilicum.

作者信息

Rampelotti-Ferreira Fátima T, Ferreira Anderson, Vendramim José D, Lacava Paulo T, Azevedo João L, Araújo Welington L

机构信息

Depto de Entomologia e Acarologia, ESALQ, USP, Piracicaba, SP, Brasil.

出版信息

Neotrop Entomol. 2010 Mar-Apr;39(2):308-10. doi: 10.1590/s1519-566x2010000200026.

Abstract

The colonization of Spodoptera frugiperda J.E. Smith larvae and rice seedlings by genetically modified endophytic bacterium Methylobacterium mesophilicum, and also the possible transfer of this bacterium to inside the larva's body during seedlings consumption were studied. The data obtained by bacterial reisolation and fluorescence microscopy showed that the bacterium colonized the rice seedlings, the larva's body and that the endophytic bacteria present in seedlings could be acquired by the larvae. In that way, the transference of endophytic bacterium from plants to insect can be a new and important strategy to insect control using engineered microorganisms.

摘要

研究了转基因内生细菌嗜中温甲基杆菌在草地贪夜蛾幼虫和水稻幼苗中的定殖情况,以及在幼虫取食幼苗过程中该细菌向幼虫体内转移的可能性。通过细菌再分离和荧光显微镜获得的数据表明,该细菌定殖在水稻幼苗和幼虫体内,且幼虫能够获取幼苗中的内生细菌。如此一来,内生细菌从植物向昆虫的转移可能成为利用工程微生物进行昆虫防治的一种新的重要策略。

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