Tohoku Research Station, Environmental/Enzootic Diseases Research Team, National Institute of Animal Health, 31 Uminai, Shichinohe, Kamikita, Aomori 039-2586, Japan.
J Food Prot. 2010 May;73(5):957-9. doi: 10.4315/0362-028x-73.5.957.
This study reports the preliminary evaluation of flow-through immunocapture (FTI) followed by real-time PCR (FTI-PCR) for the detection of different serotypes of Salmonella in pig fecal specimens. The FTI-PCR method was compared with the direct plating of FTI beads on xylose lysine desoxycholate agar, real-time PCR, and the conventional culturing methods, Rappaport-Vassiliadis and modified semisolid Rappaport-Vassiliadis. Artificially contaminated swine fecal specimens were used to evaluate and compare these methods. The results of our comparisons indicate that the FTI-PCR, FTI-plating, and modified semisolid Rappaport-Vassiliadis culture methods were accurate, specific, and sensitive. Moreover, FTI-PCR was the fastest method, providing results in fewer than 20 h, as opposed to the 48 to 96 h required for the other methods. Our results indicate that FTI-PCR could be a useful tool for detecting Salmonella in swine fecal specimens at a lower limit of 1.0 CFU/g.
本研究报告了基于流穿免疫捕获（FTI）的实时 PCR（FTI-PCR）方法，用于检测猪粪便样本中的不同血清型沙门氏菌。FTI-PCR 方法与 FTI 珠在木糖赖氨酸去氧胆酸盐琼脂上的直接平板培养、实时 PCR 和传统培养方法（Rappaport-Vassiliadis 和改良半固体 Rappaport-Vassiliadis）进行了比较。使用人工污染的猪粪便标本对这些方法进行了评估和比较。我们的比较结果表明，FTI-PCR、FTI 平板培养和改良半固体 Rappaport-Vassiliadis 培养方法准确、特异、敏感。此外，FTI-PCR 是最快的方法，不到 20 小时即可得出结果，而其他方法则需要 48 至 96 小时。我们的结果表明，FTI-PCR 可以作为一种有用的工具，用于检测猪粪便样本中沙门氏菌的含量下限为 1.0 CFU/g。
