Comparison between a cultural procedure using Rappaport-Vassiliadis broth and motility enrichments on modified semisolid Rappaport-Vassiliadis medium for Salmonella detection from food and feed.

Motility enrichment on modified semisolid Rappaport-Vassiliadis (MSRV) medium was evaluated for a rapid Salmonella detection in naturally contaminated food- and feed samples. The results of this procedure were compared with a conventional cultural procedure using Rappaport-Vassiliadis (RV) broth as selective enrichment and modified brilliant green-, xylose lysine desoxycholate- and mannitol lysine crystal violet brilliant green agar for selective plating. The productivity of motility enrichment was 87% compared to a productivity of 99% for the cultural procedure. Statistical analysis showed that there was a significant difference at the 5% level. The present study shows that a combination of direct motility enrichment with the cultural procedure could be used as a very rapid and highly cost-efficient method for Salmonella detection.