川芎嗪对成年大鼠局灶性脑缺血后的神经保护作用及神经发生增强作用
Neuroprotection and enhanced neurogenesis by tetramethylpyrazine in adult rat brain after focal ischemia.
作者信息
Xiao Xinli, Liu Yong, Qi Cunfang, Qiu Fen, Chen Xinlin, Zhang Jianshui, Yang Pengbo
机构信息
Institute of Neurobiology, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, State Key Subject for Physiology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
出版信息
Neurol Res. 2010 Jun;32(5):547-55. doi: 10.1179/174313209X414533.
OBJECTIVE
To investigate the effects of tetramethylpyrazine (TMP) on neural cell proliferation and differentiation in brain of rat after focal cerebral ischemia.
METHODS
The focal cerebral ischemia of rat was induced by middle cerebral artery occlusion (MCAO). Infarction volume was evaluated by TTC staining method. Immunohistochemistry was used to identify proliferating and differentiating cells.
RESULTS
TMP protected brain from damage by reducing volume of infarction, neuronal loss and water content. TMP not only increased the number of BrdU positive cell in SVZ, but also stimulated the cell differentiation after ischemia. The nNOS expression in cortex and dentate gyrus was reduced by treatment of TMP.
CONCLUSION
These results indicate that TMP could protect ischemic brain damage, and promote cell proliferation and differentiation stimulated by ischemia, which might be related to the reduction of nNOS expression after treatment with TMP in rat cerebral ischemia model.
目的
探讨川芎嗪（TMP）对局灶性脑缺血大鼠脑内神经细胞增殖和分化的影响。
方法
采用大脑中动脉闭塞（MCAO）法诱导大鼠局灶性脑缺血。通过TTC染色法评估梗死体积。采用免疫组织化学法鉴定增殖和分化细胞。
结果
TMP通过减少梗死体积、神经元丢失和含水量来保护脑损伤。TMP不仅增加了室管膜下区（SVZ）BrdU阳性细胞的数量，还刺激了缺血后的细胞分化。TMP治疗可降低皮质和齿状回中nNOS的表达。
结论
这些结果表明，TMP可保护缺血性脑损伤，促进缺血刺激的细胞增殖和分化，这可能与TMP治疗大鼠脑缺血模型后nNOS表达降低有关。
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