豚鼠肠肌间神经丛神经元中速激肽（P物质、神经激肽A和神经肽K）的生物合成

Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus.

作者信息

Deacon C F, Conlon J M

机构信息

Clinical Research Group for Gastrointestinal Endocrinology, Max-Planck-Gesellschaft, University of Göttingen, Gosslerstrasse 10D, D-3400 Göttingen, F.R.G.

出版信息

Neurochem Int. 1987;10(4):559-64. doi: 10.1016/0197-0186(87)90085-4.

DOI:10.1016/0197-0186(87)90085-4

PMID:20501131

Abstract

Incubation of the myenteric plexus-containing longitudinal muscle layer of guinea pig small intestine with [(35)S]methionine for up to 9 h resulted in a progressive increase in the incorporation of radioactivity into newly synthesized substance P, neurokinin A and neuropeptide K. The radiolabelled peptides were isolated from tissue extracts by immunoprecipitation using regionally-specific antisera and purified by reverse-phase high performance liquid chromatography. Incorporation of radioactivity into the tachykinins was abolished by cycloheximide and was not observed when [(35)S]cysteine was substituted for [(35)S]methionine. The method may be used to study the regulation of biosynthesis and posttranslational modification of protachykinins in the gut.

摘要

用[³⁵S]蛋氨酸孵育豚鼠小肠含肌间神经丛的纵行肌层长达9小时，导致新合成的P物质、神经激肽A和神经肽K中放射性掺入量逐渐增加。通过使用区域特异性抗血清进行免疫沉淀从组织提取物中分离出放射性标记的肽，并通过反相高效液相色谱法进行纯化。放线菌酮可消除放射性掺入速激肽的过程，并且当用[³⁵S]半胱氨酸替代[³⁵S]蛋氨酸时未观察到这种掺入。该方法可用于研究肠道中前速激肽生物合成和翻译后修饰的调控。