Drejer J, Frandsen A, Honoré T, Schousboe A
A/S Ferrosan, Research Division, Department of Biochemistry, Sydmarken 5, DK-2860 Soeborg, Denmark.
Neurochem Int. 1987;11(1):77-81. doi: 10.1016/0197-0186(87)90151-3.
The release of [(3)H]d-aspartate from cultured cerebellar granule cells (a glutamatergic neuron) was studied. It was found that the l-glutamate induced stimulation of [(3)H]d-aspartate release from the neurons could be decreased 30-40% by adenosine, cyclohexyladenosine and (?)N(6)-(2-phenylisopropyl)-adenosine all in the dose range 1-10 ?M. The effects of adenosine agonists could be blocked and even reversed by the adenosine antagonist theophyllin indicating a possible endogenous adenosine tonus on the neurons. The results add further evidence to the notion that adenosine A(1) receptors present on parallel fiber terminals in the cerebellar cortex are involved in the regulation of glutamate transmitter release from the nerve terminals.
研究了培养的小脑颗粒细胞（一种谷氨酸能神经元）中[（3）H] -d-天冬氨酸的释放。结果发现，在1 - 10 μM的剂量范围内，腺苷、环己基腺苷和（？）N（6）-（2-苯异丙基）-腺苷均可使l-谷氨酸诱导的神经元[（3）H] -d-天冬氨酸释放刺激减少30 - 40%。腺苷拮抗剂茶碱可阻断甚至逆转腺苷激动剂的作用，这表明神经元上可能存在内源性腺苷张力。这些结果进一步证明了小脑皮质平行纤维终末上存在的腺苷A（1）受体参与神经终末谷氨酸递质释放调节的观点。
