Butcher S P, Hagberg H, Sandberg M, Hamberger A
Institute of Neurobiology, University of Goteberg, Sweden.
Neurochem Int. 1987;11(1):95-9. doi: 10.1016/0197-0186(87)90154-9.
The effects of d-2-amino-5-phosphonovalerate on severe hypoglycemia-induced alterations in extracellular levels of amino acids and purine catabolites were examined in the rat striatum using the brain microdialysis technique. The extracellular concentrations of purine catabolites (hypoxanthine, xanthine, inosine and adenosine) increased dramatically during the isoelectric period. Following readministration of glucose, adenosine levels fell to control levels. The concentration of the other purine catabolites continued to rise to a maximum 20-40 min later. Although the concentration of hypoxanthine and inosine was reduced after 50-70 min, xanthine efflux remained elevated. Perfusion of d-2-amino-5-phosphonovalerate (1 mM) via a dialysis probe did not affect either the efflux of purine catabolites during the isoelectric period, or the maximum response observed during the recovery period. However, in d-2-amino-5-phosphonovalerate perfused striata the overflow of hypoxanthine, xanthine and inosine was significantly lower 50-70 min after glucose administration. d-2-amino-5-phosphonovalerate perfusion did not affect the extracellular overflow of amino acids during the isolectric period.
使用脑微透析技术，在大鼠纹状体中研究了d-2-氨基-5-磷酸戊酸对严重低血糖诱导的细胞外氨基酸和嘌呤分解代谢物水平变化的影响。在等电期，嘌呤分解代谢物（次黄嘌呤、黄嘌呤、肌苷和腺苷）的细胞外浓度急剧增加。再次给予葡萄糖后，腺苷水平降至对照水平。其他嘌呤分解代谢物的浓度在20 - 40分钟后继续上升至最高值。尽管50 - 70分钟后次黄嘌呤和肌苷的浓度降低，但黄嘌呤流出量仍保持升高。通过透析探针灌注d-2-氨基-5-磷酸戊酸（1 mM）既不影响等电期嘌呤分解代谢物的流出，也不影响恢复期观察到的最大反应。然而，在灌注d-2-氨基-5-磷酸戊酸的纹状体中，给予葡萄糖50 - 70分钟后，次黄嘌呤、黄嘌呤和肌苷的溢出量显著降低。d-2-氨基-5-磷酸戊酸灌注在等电期不影响氨基酸的细胞外溢出。
