赖氨酸向培养的大鼠星形胶质细胞和神经胶质瘤细胞的转运。

Transport of lysine into cultured rat astrocytes and glioma cells.

作者信息

Mokrasch L C

机构信息

Department of Biochemistry and Molecular Biology, LSU Medical Center, New Orleans, LA 70119, U.S.A.

出版信息

Neurochem Int. 1987;11(3):305-11. doi: 10.1016/0197-0186(87)90051-9.

DOI:10.1016/0197-0186(87)90051-9

PMID:20501176

Abstract

Cultured confluent rat astrocytes and C6 rat astrocytoma cells transport lysine with similar kinetic parameters. More than one transport mechanism may exist as suggested by discontinuities in the kinetic parameters. Sodium ion has a dual effect, being inhibitory between 1 and 10 mM and stimulatory between 30 and 137 mM. Exchange transport appears to be a minor component of lysine influx. The rate of transport into confluent cells is also affected by the recency of culture medium replacement, probably reflecting hormonal influences.

摘要

培养的汇合大鼠星形胶质细胞和C6大鼠星形胶质细胞瘤细胞以相似的动力学参数转运赖氨酸。动力学参数的不连续性表明可能存在不止一种转运机制。钠离子具有双重作用，在1至10 mM之间起抑制作用，在30至137 mM之间起刺激作用。交换转运似乎是赖氨酸流入的次要组成部分。转运到汇合细胞中的速率也受培养基更换时间的影响，这可能反映了激素的影响。