关于正常人和阿尔茨海默病患者供体的成纤维细胞中胆碱转运增强的研究。

Studies on choline transport enhancement into fibroblasts from normals and Alzheimer's donors.

作者信息

Mokrasch L C

机构信息

Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70119.

出版信息

Neurochem Res. 1991 Jul;16(7):757-61. doi: 10.1007/BF00965684.

DOI:10.1007/BF00965684

PMID:1944764

Abstract

Human dental fibroblasts transport choline actively. This transport is inhibitable by hemicholinium-3. In this paper, choline transport into fibroblasts of normal donors (four cell lines) and into those of Alzheimer victims (four cell lines, age and sex matched to the normals) is accelerated by methylated xanthines, nicotine, and dexamethasone. At a caffeine concentration of 10 microM the stimulation of choline transport into normal cells averages 128% and into Alzheimer donor cells, 45%. 1 microM Dexamethasone stimulates choline influx by 86% in normal cells and 36% in Alzheimer cells. Nicotine enhances choline transport by 35% in normal cells and by 16% in Alzheimer cells. The implication is that if Alzheimer's disease is a cholinergic disorder, it may be amenable to transport-directed chemotherapies.

摘要

人牙龈成纤维细胞能主动转运胆碱。这种转运可被半胱氨酸-3抑制。在本文中，甲基化黄嘌呤、尼古丁和地塞米松可加速胆碱向正常供体（四个细胞系）和成纤维细胞以及阿尔茨海默病患者（四个细胞系，年龄和性别与正常人匹配）的成纤维细胞的转运。在咖啡因浓度为10微摩尔时，胆碱向正常细胞转运的刺激平均为128%，向阿尔茨海默病供体细胞的刺激为45%。1微摩尔地塞米松可使正常细胞中胆碱流入增加86%，在阿尔茨海默病细胞中增加36%。尼古丁可使正常细胞中胆碱转运增加35%，在阿尔茨海默病细胞中增加16%。这意味着，如果阿尔茨海默病是一种胆碱能障碍，那么它可能适合采用针对转运的化学疗法。