肺腺癌组织中高尔基体磷蛋白 2（GOLPH2）表达上调。
Up-regulated Golgi phosphoprotein 2 (GOLPH2) expression in lung adenocarcinoma tissue.
机构信息
State Key Laboratory of Respiratory Diseases, Laboratory of Viral Immunology, Guangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou 510530, China.
出版信息
Clin Biochem. 2010 Aug;43(12):983-91. doi: 10.1016/j.clinbiochem.2010.05.010. Epub 2010 May 24.
OBJECTIVES
The aim of the study was to evaluate Golgi Phosphoprotein 2 (GOLPH2) expression in lung cancer.
DESIGN AND METHODS
GOLPH2 expression was analyzed from 178 lung cancer tumor tissue samples using immunohistochemistry. Levels of 133 lung cancer patients' serum GOLPH2 (sGOLPH2) were also quantitatively compared with 70 healthy individuals by a sandwich enzyme-linked immunosorbent assay (ELISA).
RESULTS
All adenocarcinoma (ADC) tissue samples (100%) displayed strong staining, 83.6% of which displayed cytoplasmic diffused staining. However, for squamous cell carcinoma samples, only 16.3% displayed strong staining intensity. Statistically, GOLPH2 expression in tissues of ADC was significantly higher than in other types of lung cancer (P<0.001). Levels of sGOLPH2 were also detected. The mean value of sGOLPH2 concentration is 69 ng/ml in lung cancer patients and 53 ng/ml in healthy individuals.
CONCLUSIONS
Significantly elevated GOLPH2 expression was observed in ADC tumor tissues. The levels of sGOLPH2 were about 30% higher in lung cancer patients compared with healthy individuals.
目的
本研究旨在评估肺癌中高尔基磷蛋白 2（GOLPH2）的表达。
设计和方法
采用免疫组织化学法分析 178 例肺癌肿瘤组织样本中的 GOLPH2 表达。通过夹心酶联免疫吸附试验（ELISA）定量比较 133 例肺癌患者血清 GOLPH2（sGOLPH2）水平与 70 例健康个体。
结果
所有腺癌（ADC）组织样本（100%）均显示强染色，其中 83.6%显示细胞质弥漫染色。然而，对于鳞状细胞癌样本，仅有 16.3%显示强染色强度。统计学分析显示，ADC 组织中的 GOLPH2 表达明显高于其他类型的肺癌（P<0.001）。还检测了 sGOLPH2 的水平。肺癌患者 sGOLPH2 浓度的平均值为 69ng/ml，健康个体为 53ng/ml。
结论
在 ADC 肿瘤组织中观察到 GOLPH2 表达明显升高。与健康个体相比，肺癌患者的 sGOLPH2 水平约高 30%。
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