[肝细胞癌患者的微小RNA分析]
[MicroRNA profiling in patients with hepatocellular carcinoma].
作者信息
Wang Chuan-xi, Song Wei, Li Zeng-jun, Song Bao, Wu De-hua, Sun Ai-min, Chen Long-hua
机构信息
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):976-7.
OBJECTIVE
To investigate the differential expression of microRNAs between human hepatocellular carcinoma (HCC) and liver cirrhosis (LC).
METHODS
The total RNA was extracted from 25 pairs of HCC and LC tissues. microRNA microarray was used to analyze the microRNA expression profiles, and validation of the microarray results was carried out by real-time quantitative RT-PCR (qRT-PCR).
RESULTS
Three microRNAs exhibited higher expression in the HCC samples than in the LC samples. In comparison with the LC samples, the HCC samples showed down-regulated expressions of 9 microRNAs. qRT-PCR verified that has-miR-122a, has-miR-199a, and has-miR-199b were downregulated in HCC, which was in agreement with the microarray results.
CONCLUSION
HCC and LC samples have significantly different microRNA expression profiles. These differentially expressed microRNAs may be involved in the pathogensis of HCC.
目的
研究人类肝细胞癌（HCC）与肝硬化（LC）之间微小RNA的差异表达。
方法
从25对HCC和LC组织中提取总RNA。采用微小RNA芯片分析微小RNA表达谱，并通过实时定量逆转录聚合酶链反应（qRT-PCR）对芯片结果进行验证。
结果
三种微小RNA在HCC样本中的表达高于LC样本。与LC样本相比，HCC样本中有9种微小RNA表达下调。qRT-PCR验证了has-miR-122a、has-miR-199a和has-miR-199b在HCC中表达下调，这与芯片结果一致。
结论
HCC和LC样本具有显著不同的微小RNA表达谱。这些差异表达的微小RNA可能参与了HCC的发病机制。
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