不同浓度伊马替尼对大鼠C6胶质瘤细胞凋亡及细胞周期的影响

[Effect of imatinib at different concentrations on rat C6 glioma cell apoptosis and cell cycle].

作者信息

Yang Ling, Xu Zhong-yuan, Chen Xiao-hua, Wang Ke-wan, Li Guo-feng, Chen Zhi-liang

机构信息

Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):1089-91.

PMID:20501402

Abstract

OBJECTIVE

To investigate the effect of imatinib on rat C6 glioma cell apoptosis and cell cycle.

METHODS

MTT assay was used to determine the OD value of C6 glioma cells following treatment with imatinib at different concentrations (0.156, 10 and 15 micromo/L) for 24, 48 and 72 h. The cell apoptosis was assayed by Hochest/PI staining and the cell cycle changes were analyzed by flow cytometry.

RESULTS

Imatinib treatment resulted in increased number of apoptotic cells in a time- and dose-dependent manner. A 72-h treatment of the cells with imatinib at 10 and 15 micromo/L caused increased cell percentage in G(0)/G(1) phase to (68.53-/+0.83)% and (70.41-/+0.62)%, (P<0.01), decreased the percentage of G(2) phase cells to (14.48-/+0.12)% and (13.84-/+2.86)% (P<0.01), and decreased the percentage of S phase cells to (16.98-/+0.72)% and (15.78-/+2.28)%, respectively (P<0.01).

CONCLUSION

Imatinib can induce apoptosis and affect the distribution of the cell cycle of C6 cells in vitro.

摘要

目的

探讨伊马替尼对大鼠C6胶质瘤细胞凋亡及细胞周期的影响。

方法

采用MTT法检测不同浓度（0.156、10和15微摩尔/升）伊马替尼作用24、48和72小时后C6胶质瘤细胞的光密度值。通过Hochest/PI染色检测细胞凋亡，采用流式细胞术分析细胞周期变化。

结果

伊马替尼处理导致凋亡细胞数量呈时间和剂量依赖性增加。用10和15微摩尔/升伊马替尼处理细胞72小时后，G(0)/G(1)期细胞百分比增加至(68.53±0.83)%和(70.41±0.62)%（P<0.01），G(2)期细胞百分比降至(14.48±0.12)%和(13.84±2.86)%（P<0.01），S期细胞百分比分别降至(16.98±0.72)%和(15.78±2.28)%（P<0.01）。