Jiang Qun, Tu Wei-feng
Postgraduate School, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):1099-102.
To investigate the effects of sufentanil pretreatment on acute gastric mucosal lesion and its impact on the expression of acid-sensing ion channel 3 (ASIC3) in thoracic dorsal root ganglia (DRG) neurons in rats with water immersion-restraint stress (WIRS).
Twenty-four Wistar rats were randomly assigned into 3 groups, namely the normal control group (n=6), WIRS group (n=12) and sufentanil pretreatment group (n=6). Gastric mucosal lesion was induced by WIRS, and after 6 h of WIRS, the gastric tissues were excised and observed under microscope, with the ulcer index (UI) calculated. The expression of ASIC3 in the DRG neurons was detected by immunofluorescence assay, and the ASIC3 mRNA expression by quantitative real-time RT-PCR.
Compared with the normal control group, the rats in the WIRS group showed obvious gastric injury with increased UI and extensive expression of ASIC3 in the DRG neurons. Sufentanil pretreatment of the rats subjected to WIRS significantly alleviated the gastric mucosal injury, lowered the UI, and reduced ASIC3 mRNA expression in thoracic DRG neurons.
ASIC3 is involved in the development of acute gastric mucosal lesion, and sufentanil pretreatment offers protection of gastric mucosa by inhibiting the expression of ASIC3.
探讨舒芬太尼预处理对水浸束缚应激（WIRS）大鼠急性胃黏膜损伤的影响及其对胸段背根神经节（DRG）神经元中酸敏感离子通道3（ASIC3）表达的影响。
将24只Wistar大鼠随机分为3组，即正常对照组（n = 6）、WIRS组（n = 12）和舒芬太尼预处理组（n = 6）。通过WIRS诱导胃黏膜损伤，WIRS 6小时后，切除胃组织并在显微镜下观察，计算溃疡指数（UI）。采用免疫荧光法检测DRG神经元中ASIC3的表达，采用实时定量RT-PCR检测ASIC3 mRNA的表达。
与正常对照组相比，WIRS组大鼠胃损伤明显，UI升高，DRG神经元中ASIC3广泛表达。对WIRS大鼠进行舒芬太尼预处理可显著减轻胃黏膜损伤，降低UI，并降低胸段DRG神经元中ASIC3 mRNA的表达。
ASIC3参与急性胃黏膜损伤的发生发展，舒芬太尼预处理通过抑制ASIC3的表达对胃黏膜起到保护作用。
