Bhusari K P, Khedekar P B, Dhole Seema, Banode V S
Department of Pharmaceutical Chemistry, Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur-441 110, India.
Indian J Pharm Sci. 2009 Sep;71(5):505-8. doi: 10.4103/0250-474X.58176.
Two methods for simultaneous estimation of hydrochlorothiazide and olmesartan medoxomil in combined tablet dosage form have been developed. The first method is the application of Q-analysis method (absorbance ratio), which involves the formation of Q-absorbance equation at 264 nm (isobestic point) and at 271 nm, the maximum absorption of hydrochlorothiazide. The linearity ranges for hydrochlorothiazide and olmesartan medoxomil were 2.5-22.5 mug/ml and 4-36 mug/ml, respectively. The second method is based on the derivative spectrophotometric method at zero crossing wavelengths. The linearity ranges for hydrochlorothiazide and olmesartan medoxomil were 2.5-20 mug/ml and 4-32 mug/ml, respectively. The accuracy of the methods were assessed by recovery studies and was found to be 100.45% +/-0.4215 and 100.24% +/-0.3783 for absorbance ratio method and 99.39% +/-0.221 and 99.72% +/-0.11 for first derivative method, for hydrochlorothiazide and olmesartan medoxomil, respectively. These methods are simple, accurate and rapid, those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.
已开发出两种同时测定复方片剂剂型中氢氯噻嗪和奥美沙坦酯含量的方法。第一种方法是应用Q分析方法（吸光度比），该方法涉及在264nm（等吸收点）和271nm（氢氯噻嗪的最大吸收波长）处形成Q吸光度方程。氢氯噻嗪和奥美沙坦酯的线性范围分别为2.5 - 22.5μg/ml和4 - 36μg/ml。第二种方法基于零交叉波长处的导数分光光度法。氢氯噻嗪和奥美沙坦酯的线性范围分别为2.5 - 20μg/ml和4 - 32μg/ml。通过回收率研究评估了这些方法的准确性，发现对于吸光度比法，氢氯噻嗪和奥美沙坦酯的回收率分别为100.45%±0.4215和100.24%±0.3783；对于一阶导数法，氢氯噻嗪和奥美沙坦酯的回收率分别为99.39%±0.221和99.72%±0.11。这些方法简单、准确、快速，无需预先分离，因此可用于质量控制实验室对两种药物的常规分析。
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