Dinç Erdal, Baleanu Dumitru
Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, 06100, Tandoğan, Ankara, Turkey.
J Pharm Biomed Anal. 2002 Oct 15;30(3):715-23. doi: 10.1016/s0731-7085(02)00359-x.
Four chemometric methods were applied to simultaneous determination of cilazapril and hydrochlorothiazide in tablets. Classical least-square (CLS), inverse least-square (ILS), principal component regression (PCR) and partial least-squares (PLS) methods do not need any priori graphical treatment of the overlapping spectra of two drugs in a mixture. For all chemometric calibrations a concentration set of the random mixture consisting of the two drugs in 0.1 M HCI and methanol (1:1) was prepared. The absorbance data in the UV-Vis spectra were measured for the 15 wavelength points (from 222 to 276 nm) in the spectral region 210-290 nm considering the intervals of deltalambda = 4 nm. The calibration of the investigated methods involves only absorbance and concentration data matrices. The developed calibrations were tested for the synthetic mixtures consisting of two drugs and using the Maple V software the chemometric calculations were performed. The results of the methods were compared each other as well as with HPLC method and a good agreement was found.
采用四种化学计量学方法同时测定片剂中西拉普利和氢氯噻嗪的含量。经典最小二乘法（CLS）、逆最小二乘法（ILS）、主成分回归法（PCR）和偏最小二乘法（PLS）无需对混合物中两种药物的重叠光谱进行任何先验的图形处理。对于所有化学计量学校准，制备了由两种药物在0.1 M盐酸和甲醇（1:1）中组成的随机混合物的浓度集。考虑到δλ = 4 nm的间隔，在210 - 290 nm光谱区域内的15个波长点（从222到276 nm）测量紫外-可见光谱中的吸光度数据。所研究方法的校准仅涉及吸光度和浓度数据矩阵。对由两种药物组成的合成混合物进行所开发校准的测试，并使用Maple V软件进行化学计量学计算。将这些方法的结果相互比较以及与高效液相色谱法比较，发现结果吻合良好。
