Fratté S, Gendrault J L, Steffan A M, Kirn A
Groupe de Recherches de l'INSERM (U 74), Université Louis Pasteur, Strasbourg, France.
Hepatology. 1991 Jun;13(6):1173-80.
University of Wisconsin solution greatly lengthens the time liver storage is possible compared with all previous solutions used. To test whether this improvement is related to better preservation of the endothelial cell, which is thought to be the most vulnerable cell type in cold storage, we compared time-related ultrastructural changes in rat livers stored in this solution or in Euro-Collins solution. Rat livers were harvested after combined arterial and portal perfusion with the cold-storage solution. They were then preserved for different lengths of time in the same solution at 4 degrees C before being perfusion-fixed and processed for light and electron microscopy. The first preservation damage was noted in endothelial cells; the time course of the lesions was similar in both solutions. After 2 hr of storage, enlarged and ruptured fenestrae with many gaps were observed. Swollen at 4 hr, the endothelial cells became stringlike at 10 hr, leading to stripped sinusoidal walls. Hepatocytes appeared better preserved in University of Wisconsin solution. The amount of glycogen, maintained near the control level at 24 hr in the latter, decreased dramatically between 0 and 4 hr in Euro-Collins solution, as ultrastructurally observed and biochemically confirmed. Furthermore, sinusoidal obstruction by blebs originating from the hepatocytes and quantified by image analysis on electron micrographs was markedly delayed. It was significantly less pronounced in University of Wisconsin solution at 24 hr than in Euro-Collins solution at 2 hr (p less than or equal to 0.05). Our findings confirm that endothelial cells are highly susceptible to preservation damage and show that University of Wisconsin solution does not improve preservation during storage.(ABSTRACT TRUNCATED AT 250 WORDS)
与之前使用的所有溶液相比，威斯康星大学溶液大大延长了肝脏保存的时间。为了测试这种改善是否与内皮细胞的更好保存有关，内皮细胞被认为是冷藏中最脆弱的细胞类型，我们比较了储存在该溶液或欧洲柯林斯溶液中的大鼠肝脏随时间的超微结构变化。大鼠肝脏在联合动脉和门静脉灌注冷保存溶液后收获。然后将它们在相同溶液中于4℃保存不同时间长度，之后进行灌注固定并处理用于光镜和电镜检查。首先观察到的保存损伤在内皮细胞；两种溶液中病变的时间进程相似。储存2小时后，观察到窗孔扩大和破裂并有许多间隙。4小时时内皮细胞肿胀，10小时时变成线状，导致肝血窦壁剥离。在威斯康星大学溶液中肝细胞似乎保存得更好。糖原含量在后者中24小时时维持在接近对照水平，而在欧洲柯林斯溶液中在0至4小时之间显著下降，这通过超微结构观察和生化证实。此外，由肝细胞产生的小泡引起的肝血窦阻塞并通过电子显微镜图像分析定量，明显延迟。在24小时时威斯康星大学溶液中的这种阻塞明显不如2小时时欧洲柯林斯溶液中的明显（p小于或等于0.05）。我们的发现证实内皮细胞对保存损伤高度敏感，并表明威斯康星大学溶液在储存期间并没有改善保存效果。（摘要截短于250字）
