Department of Genetics, Case Western Reserve University, Cleveland, Ohio, USA.
Dev Dyn. 2010 Jun;239(6):1755-67. doi: 10.1002/dvdy.22279.
The six mammalian CCN genes (Cyr61, CTGF, Nov, WISP1, WISP2, WISP3) encode a family of secreted, cysteine-rich, multimodular proteins having roles in cell proliferation, adhesion, migration, and differentiation during embryogenesis, wound healing, and angiogenesis. We used bioinformatics to identify 9 CCN genes in zebrafish (zCCNs), 6 of which have not been previously described. When compared with mammalian CCN family members, 3 were paralogs of Cyr61, 2 of CTGF, 2 of WISP1, 1 of WISP2, and 1 of WISP3. No paralog of Nov was found. In situ hybridization was performed to characterize the sites of expression of the zCCNs during early zebrafish development. zCCNs demonstrated both unique and overlapping patterns of expression, suggesting potential division of labor between orthologous genes and providing an alternate approach to gene function studies that will complement studies in mammalian models.
六种哺乳动物的 CCN 基因（Cyr61、CTGF、Nov、WISP1、WISP2、WISP3）编码了一组分泌型、富含半胱氨酸的多功能蛋白，它们在胚胎发生、创伤愈合和血管生成过程中的细胞增殖、黏附、迁移和分化中发挥作用。我们使用生物信息学方法在斑马鱼中鉴定出 9 种 CCN 基因（zCCNs），其中 6 种尚未被描述过。与哺乳动物的 CCN 家族成员相比，有 3 种是 Cyr61 的同源基因，2 种是 CTGF 的同源基因，2 种是 WISP1 的同源基因，1 种是 WISP2 的同源基因，1 种是 WISP3 的同源基因。没有发现 Nov 的同源基因。通过原位杂交技术来研究 zCCNs 在早期斑马鱼发育过程中的表达部位。zCCNs 的表达模式既有独特性又有重叠性，这表明同源基因之间可能存在分工，为基因功能研究提供了一种替代方法，可与哺乳动物模型的研究相辅相成。
