外源性神经节苷脂（G(M1)、G(D1a)、G(Mix)）对黑线毛足鼠（Phodopus sungorus）脑细胞及亚细胞组分中钙激活的镁依赖性ATP酶的影响

Influence of exogenous gangliosides (G(M1), G(D1a), G(Mix)) on a Ca(2+)-activated Mg(2+)-dependent ATPase in cellular and subcellular brain fractions of the djungarian dwarf hamster (Phodopus sungorus).

作者信息

Slenzka K, Appel R, Rahmann H

机构信息

Institute of Zoology, University of Stuttgart-Hohenheim, Garbenstrasse 30, D-7000 Suttgart 70 (Hohenheim), F.R.G.

出版信息

Neurochem Int. 1990;17(4):609-14. doi: 10.1016/0197-0186(90)90050-4.

DOI:10.1016/0197-0186(90)90050-4

PMID:20504665

Abstract

The influence of 150 nM exogenously-added gangliosides (G(M1), G(D1a), G(Mix)) on a Ca(2+)-activated, Mg(2+)-dependent ATPase was investigated in cellular and subcellular fractions (P1-fraction, synaptosomal fraction, synaptic membranes) from whole brain, cortex, cerebellum and brain stem of the djungarian dwarf hamster (Phodopus sungorus). Gangliosides are effective at this concentration in stimulating the enzyme activity in all fractions from whole brain. Inhomogenous results (stimulation, inhibition and no effects), however, were obtained in the different individual brain regions.

摘要

研究了外源性添加150 nM神经节苷脂（GM1、GD1a、混合神经节苷脂）对来自黑线毛足鼠（Phodopus sungorus）全脑、皮质、小脑和脑干的细胞及亚细胞组分（P1组分、突触体组分、突触膜）中钙激活、镁依赖的ATP酶的影响。在此浓度下，神经节苷脂可有效刺激全脑所有组分中的酶活性。然而，在不同的单个脑区获得了不均匀的结果（刺激、抑制和无影响）。