Chan W Y
Department of Anatomy, Faculty of Medicine, Chinese University of Hong Kong, Shatin.
J Anat. 1991 Apr;175:41-50.
Small tissue fragments were isolated from 5 different regions of mouse embryos at the primitive streak-stage and were grafted ectopically into the anterior eye chambers of syngeneic adult mice. Of the grafts, 22-66% became attached to the iris and were vascularised within 2-3 days. The attached grafts grew in size and formed loose aggregates of embryonic cells. During the fourth week in oculo, fully differentiated tissues representing derivatives of all three germ layers were found within the grafts. No differences in the composition of tissues derived were observed in grafts obtained from different regions of the embryo except that the endodermal grafts formed endodermal cysts only. The size of the original fragments did not affect their in oculo differentiation but smaller fragments had a lower chance of intraocular survival.
从小鼠胚胎原条期的5个不同区域分离出小组织片段，并将其异位移植到同基因成年小鼠的前房。在这些移植物中，22%-66%附着于虹膜，并在2-3天内血管化。附着的移植物体积增大，形成胚胎细胞的松散聚集体。在眼内的第四周，在移植物中发现了代表所有三个胚层衍生物的完全分化组织。除了内胚层移植物仅形成内胚层囊肿外，从胚胎不同区域获得的移植物中，所衍生组织的组成没有差异。原始片段的大小不影响其在眼内的分化，但较小的片段眼内存活的机会较低。
