Simpson R C, Emary W B, Lys I, Cotter R J, Fenselau C C
Department of Chemistry and Biochemistry, University of Maryland, Baltimore 21228.
J Chromatogr. 1991 Jan 4;536(1-2):143-53. doi: 10.1016/s0021-9673(01)89245-1.
High-performance liquid chromatography (HPLC) has been successfully interfaced on-line with liquid secondary-ion time-of-flight mass spectrometry, utilizing a continuous-flow interface. Time-of-flight mass spectrometry (TOF-MS) is a low-resolution, high-mass-range technique, compatible with extremely rapid data acquisition rates. Thus a TOF-MS system is extremely well suited for coupling with HPLC. This paper describes the interface used to couple the HPLC and TOF-MS as well as the basic operating principles of such a system. Using both standard and packed-capillary reversed-phase HPLC columns, the HPLC-TOF-MS system has been successfully used to separate and detect peptides, providing molecular weight information for the peptide analytes. Experimental data, including chromatograms (UV, reconstructed ion and selected ion) and mass spectra, are presented to demonstrate the ability of the HPLC-liquid secondary-ion TOF-MS system to resolve chromatographically analytes as well as to resolve mass spectrometrically analytes which are unresolved on the chromatographic column.
高效液相色谱法（HPLC）已成功地通过连续流动接口与液体二次离子飞行时间质谱仪进行在线联用。飞行时间质谱法（TOF-MS）是一种低分辨率、高质量范围的技术，能够实现极快的数据采集速率。因此，TOF-MS系统非常适合与HPLC联用。本文介绍了用于连接HPLC和TOF-MS的接口以及该系统的基本工作原理。使用标准填充毛细管反相HPLC柱，HPLC-TOF-MS系统已成功用于分离和检测肽段，为肽分析物提供分子量信息。文中给出了包括色谱图（紫外、重建离子和选择离子）和质谱在内的实验数据，以证明HPLC-液体二次离子TOF-MS系统在色谱上分离分析物以及在质谱上分离色谱柱上未分离的分析物的能力。
