Kitazume E, Bhatnagar M, Ito Y
Laboratory of Biophysical Chemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
J Chromatogr. 1991 Jan 18;538(1):133-40. doi: 10.1016/s0021-9673(01)91630-9.
Besides being widely used in electronic and glass industries, rare earth elements have recently been found to have important biological effects including the ability to stabilize and enhance interferon activity [J.J. Sedmak and S.E. Grossberg, J. Gen. Virol, 52 (1981) 195]. In this paper, the rare earth elements have been separated using a high-speed counter-current chromatography (HSCCC) centrifuge equipped with three multilayer coils connected in series. Two-phase solvent systems were composed of n-heptane containing di-(2-ethylhexyl)phosphoric acid (stationary phase) and dilute hydrochloric acid (mobile phase) where the partition coefficient of each can be optimized by selecting the proper hydrochloric acid concentration. The mobile phase was eluted through the column at a flow-rate of 5 ml/min, while the apparatus was rotated at 900 rpm. Continuous detection of the rare earth elements was effected by means of a post-column reaction with arsenazo III and the elution curve was obtained by on-line monitoring at 650 nm. Excellent isocratic separations of closely related rare earth elements were achieved at high partition efficiencies up to several thousand theoretical plates. Versatility of the present method was demonstrated in an exponential gradient elution of hydrochloric acid concentration where fourteen rare earth elements were all resolved in about 4.5 h.
除了在电子和玻璃工业中广泛应用外，最近发现稀土元素具有重要的生物学效应，包括稳定和增强干扰素活性的能力[J.J. 塞德马克和S.E. 格罗斯伯格，《普通病毒学杂志》，52 (1981) 195]。在本文中，使用配备三个串联多层线圈的高速逆流色谱(HSCCC)离心机对稀土元素进行了分离。两相溶剂系统由含有二(2-乙基己基)磷酸的正庚烷(固定相)和稀盐酸(流动相)组成，通过选择合适的盐酸浓度可以优化每种物质的分配系数。流动相以5 ml/min的流速通过柱子洗脱，同时仪器以900 rpm的转速旋转。通过与偶氮胂III进行柱后反应对稀土元素进行连续检测，并通过在650 nm处的在线监测获得洗脱曲线。在高达数千个理论塔板的高分配效率下，实现了密切相关稀土元素的优异等度分离。在盐酸浓度的指数梯度洗脱中证明了本方法的通用性，其中14种稀土元素在约4.5小时内全部得到分离。
