Division of Exercise Physiology, West Virginia University School of Medicine, PO Box 9227, Morgantown, WV 26506, USA.
J Gerontol A Biol Sci Med Sci. 2010 Aug;65(8):815-31. doi: 10.1093/gerona/glq080. Epub 2010 May 27.
This study tested the hypothesis that resveratrol supplementation would lower oxidative stress in exercised muscles of aged mice. Young (3 months) and aged (27 months) C57BL/6 mice received a control or a 0.05% trans-resveratrol-supplemented diet for 10 days. After 7 days of dietary intervention, 20 maximal electrically evoked isometric contractions were obtained from the plantar flexors of one limb in anesthetized mice. Exercise was conducted for three consecutive days. Resveratrol supplementation blunted the exercise-induced increase in xanthine oxidase activity in muscles from young (25%) and aged (53%) mice. Resveratrol lowered H(2)O(2) levels in control (13%) and exercised (38%) muscles from aged animals, reduced Nox4 protein in both control and exercised muscles of young (30%) and aged mice (40%), and increased the ratio of reduced glutathione to oxidized glutathione in exercised muscles from young (38%) and aged (135%) mice. Resveratrol prevented the increase in lipid oxidation, increased catalase activity, and increased MnSOD activity in exercised muscles from aged mice. These data show that dietary resveratrol suppresses muscle indicators of oxidative stress in response to isometric contractions in aged mice.
本研究旨在验证这样一个假设，即白藜芦醇补充剂可降低老年小鼠运动肌肉的氧化应激。将年轻（3 个月）和年老（27 个月）C57BL/6 小鼠分别喂食对照或 0.05%反式白藜芦醇补充饲料 10 天。在饮食干预 7 天后，在麻醉的小鼠的跖屈肌中获得一条肢体的 20 次最大电诱发等长收缩。连续运动 3 天。白藜芦醇补充剂可减轻年轻（25%）和年老（53%）小鼠运动引起的黄嘌呤氧化酶活性增加。白藜芦醇降低了对照（13%）和运动（38%）年老动物肌肉中的 H2O2 水平，减少了年轻（30%）和年老（40%）对照和运动肌肉中的 Nox4 蛋白，并增加了年轻（38%）和年老（135%）运动肌肉中还原型谷胱甘肽与氧化型谷胱甘肽的比值。白藜芦醇可防止年老小鼠运动肌肉中的脂质氧化增加、过氧化氢酶活性增加和 MnSOD 活性增加。这些数据表明，饮食中的白藜芦醇可抑制老年小鼠等长收缩引起的肌肉氧化应激指标。
