芪类化合物是黄嘌呤氧化酶催化产生超氧阴离子的特异性抑制剂。
Stilbene compounds are specific inhibitors of the superoxide anion generation catalyzed by xanthine oxidase.
作者信息
Masuoka Noriyoshi
机构信息
Tsudaka-Fruit Juice Laboratory, Okayama Research-Park Incubation Center, 5303 Haga, Kita-ku, Okayama 701-1221, Japan.
出版信息
Food Chem X. 2021 Oct 28;12:100146. doi: 10.1016/j.fochx.2021.100146. eCollection 2021 Dec 30.
The inhibitory effect of xanthine oxidase (XO) reactions with stilbene compounds, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging by stilbene compounds and superoxide anion (O-) scavenging activity were examined. The inhibition of the O- generation catalyzed by XO by stilbene compounds is stronger than the effect on uric acid formation. The suppression of the O- generation with resveratrol was diminished by the addition of flavin adenine dinucleotide (FAD). The water-solubility and visible spectra (VIS) of the stilbene compounds in the presence of water-soluble flavin compounds indicated a π-π interaction between the stilbene compounds and the isoalloxazine in flavin compounds. These results indicate that stilbene compounds specifically bind the FAD site in XO so as to inhibit the O- generation. In the case of piceatannol, it is deduced that the suppression of O- generation is induced by this specific binding to the FAD site and the subsequent reduction of XO.
研究了芪类化合物对黄嘌呤氧化酶（XO）反应的抑制作用、芪类化合物对1,1-二苯基-2-苦基肼（DPPH）的清除作用以及超氧阴离子（O⁻）清除活性。芪类化合物对XO催化产生O⁻的抑制作用强于对尿酸生成的影响。添加黄素腺嘌呤二核苷酸（FAD）后，白藜芦醇对O⁻生成的抑制作用减弱。在水溶性黄素化合物存在下，芪类化合物的水溶性和可见光谱（VIS）表明芪类化合物与黄素化合物中的异咯嗪之间存在π-π相互作用。这些结果表明，芪类化合物特异性结合XO中的FAD位点，从而抑制O⁻的生成。就3,4,3',5'-四羟基芪而言，推测O⁻生成的抑制是由这种与FAD位点的特异性结合以及随后XO的还原诱导的。
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