Centro de Investigação em Genética Molecular Humana (CIGMH), Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
Nanotechnology. 2010 Jun 25;21(25):255101. doi: 10.1088/0957-4484/21/25/255101. Epub 2010 May 28.
A specific colorimetric DNA detection method based on oligonucleotide functionalized gold-silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53-a gene involved in cancer development. The AuAg-alloy-nanoprobes were then used in combination with Au-nanoprobes for a one-pot dual-colour detection strategy that allowed for the simultaneous differential detection of two distinct target sequences. This system poses an unprecedented opportunity to explore the combined use of metal nanoparticles with different composition towards the development of a multiplex one-pot colorimetric assay for DNA detection.
本文提出了一种基于寡核苷酸功能化金银合金纳米粒子（AuAg-alloy-nanoprobes）的特定比色 DNA 检测方法。然后，将 AuAg-alloy-nanoprobes 用于特定检测来自 TP53-a 基因的 DNA 序列，该基因与癌症的发展有关。然后，将 AuAg-alloy-nanoprobes 与 Au 纳米探针结合使用，形成一种一锅法双色检测策略，可同时对两种不同的靶序列进行差分检测。该系统为探索使用不同组成的金属纳米粒子组合开发用于 DNA 检测的多重一锅比色测定法提供了前所未有的机会。
