Duadi Hamootal, Margalit Ofer, Zalevsky Zeev, Sarafis Vassilios
Engineering School, Bar-Ilan University, Ramat-Gan 52900, Israel.
J Opt Soc Am A Opt Image Sci Vis. 2010 Jun 1;27(6):1378-84. doi: 10.1364/JOSAA.27.001378.
In imaging systems and especially in confocal microscopy systems there is a trade-off between the lateral resolution and the obtained depth of focus. The use of complex pupils to improve the lateral resolution by engineering the point spread function is a common approach; however the lateral improvement reduces the effective depth of focus and therefore the fluorescence efficiency. In this work we analytically develop an optimized approach for obtaining a complex pupil with an extended depth of focus. The proposed solution is numerically applied and tested in designing an improved focal depth in confocal microscope configuration.
在成像系统中，尤其是在共聚焦显微镜系统中，横向分辨率和所获得的焦深之间存在权衡。通过设计点扩散函数来使用复杂光瞳以提高横向分辨率是一种常见方法；然而，横向分辨率的提高会降低有效焦深，进而降低荧光效率。在这项工作中，我们通过分析开发了一种用于获得具有扩展焦深的复杂光瞳的优化方法。所提出的解决方案在设计共聚焦显微镜配置中的改进焦深时进行了数值应用和测试。
