猴病毒40染色质，显示核小体沿着延伸的闭环DNA呈线性珠状排列。

Simian virus 40 chromatin showing nucleosomes in linear bead-like arrangements along extended closed circular DNA.

作者信息

Oda T, Watanabe S, Nakamura T, Hidaka H

出版信息

Acta Med Okayama. 1977 Dec;31(6):405-9.

PMID:205093

Abstract

Viral nucleoprotein complexes were extracted from nuclei of permissive cells (CV-1) infected with simian virus 40 (SV40) and examined by electron microscopy. SV40 nucleoprotein complexes (SV40 chromatin) showed nucleosomes in linear bead-like arrangements along the extended closed circular DNA. The contour length of the SV40 chromatin was only 1.0-1.8 times shorter than that of viral DNA obtained after deproteinization. The data suggest that the circular DNA in SV40 chromatin can be extended to nearly its full length without detachment of the histone complexes.

摘要

从感染了猴病毒40（SV40）的允许细胞（CV-1）的细胞核中提取病毒核蛋白复合物，并通过电子显微镜进行检查。SV40核蛋白复合物（SV40染色质）显示核小体沿着延伸的闭环DNA呈线性珠状排列。SV40染色质的轮廓长度仅比脱蛋白后获得的病毒DNA短1.0-1.8倍。数据表明，SV40染色质中的环状DNA可以延伸至几乎其全长，而组蛋白复合物不会脱离。