R&D Biology, Antibióticos S.A., Avenida de Antibióticos 59-61, 24009 León, Spain.
J Biotechnol. 2010 Jul 20;148(2-3):144-6. doi: 10.1016/j.jbiotec.2010.05.004. Epub 2010 May 25.
An improved semi-industrial process for astaxanthin production by fermentation of Xanthophyllomyces dendrorhous has been developed. The culture medium was designed at the flask scale, reaching an astaxanthin cellular content of 3.0 mgg(-1) cell weight and a volumetric yield of 119 mgL(-1) broth. Astaxanthin production in flask was significantly improved by white light (4.0 mgg(-1) and 221 mgL(-1)), and by ultraviolet light (4.4 mgg(-1) and 235 mgL(-1)). The scale-up to 10- and 800-L fermentors was developed by feeding with glucose. Representative data for illuminated fermentation processes are presented and discussed at the 10-L scale, where 420 mgL(-1) (4.7 mgg(-1)) astaxanthin were produced, and the 800-L scale, with productivities of 350 mgL(-1) (4.1 mgg(-1)) astaxanthin. The purity of the astaxanthin in the broth was about 84%, with accumulation of the following carotenoids other than astaxanthin: 4% beta-carotene, 4% canthaxanthin, 5% HDCO, 1% zeaxanthin and 2% phoenicoxanthin. This technology can be easily scaled-up to an industrial application for the production of this xanthophyll widely demanded nowadays.
已经开发出一种通过发酵雨生红球藻生产虾青素的改良半工业工艺。在摇瓶规模下设计培养基，虾青素细胞含量达到 3.0 mgg(-1)细胞重量和 119 mgL(-1)培养液的体积产率。通过白光（4.0 mgg(-1)和 221 mgL(-1)）和紫外线（4.4 mgg(-1)和 235 mgL(-1)）显著提高了摇瓶中的虾青素生产。通过葡萄糖进料将规模扩大到 10-和 800-L 发酵罐。在 10-L 规模下，以 420 mgL(-1)（4.7 mgg(-1)）虾青素的产量，在 800-L 规模下，以 350 mgL(-1)（4.1 mgg(-1)）虾青素的生产力，提出并讨论了光照发酵过程的代表性数据。发酵液中虾青素的纯度约为 84%，除虾青素外还积累了以下类胡萝卜素：4%β-胡萝卜素、4%角黄素、5%HDCO、1%玉米黄质和 2%叶黄素。这项技术可以很容易地扩展到工业应用，以生产当今广泛需求的这种叶黄素。
