Liu Z-Q, Zhang J-F, Zheng Y-G, Shen Y-C
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, People's Republic of China.
J Appl Microbiol. 2008 Mar;104(3):861-72. doi: 10.1111/j.1365-2672.2007.03603.x. Epub 2007 Oct 22.
Isolation, characterization and identification of Phaffia sp. ZJB 00010, and improvement of astaxanthin production with low-energy ion beam implantation.
A strain of ZJB 00010, capable of producing astaxanthin, was isolated and identified as Phaffia rhodozyma, based on its physiological and biochemical characteristics as well as its internal transcribed spacer (ITS) rDNA gene sequence analysis. With low-energy ion beam implantation, this wild-type strain was bred for improving the yield of astaxanthin. After ion beam implantation, the best mutant, E5042, was obtained. The production of astaxanthin in E5042 was 2512 microg g(-1) (dry cell weight, DCW), while the wild-type strain was about 1114 microg g(-1) (DCW), an increase of 125.5%. Moreover, the fermentation conditions of mutant E5042 for producing astaxanthin were optimized. The astaxanthin production under the optimized conditions was upscaled and studied in a 50-l fermentor.
A genetically stable mutant strain with high yield of astaxanthin was obtained using low-energy ion beam implantation. This mutant may be a suitable candidate for the industrial-scale production of astaxanthin.
Astaxanthin production in Phaffia rhodozyma could be efficiently improved by low-energy ion beam implantation, which is a new technology in the mutant breeding of micro-organisms. The mutant obtained in this work could potentially be utilized in industrial production of astaxanthin.
分离、鉴定红法夫酵母ZJB 00010，并通过低能离子束注入提高虾青素产量。
基于其生理生化特性及内转录间隔区（ITS）rDNA基因序列分析，分离得到一株能够产生虾青素的ZJB 00010菌株，并鉴定为红发夫酵母。通过低能离子束注入对该野生型菌株进行育种以提高虾青素产量。离子束注入后，获得了最佳突变体E5042。E5042中虾青素产量为2512 μg g⁻¹（干细胞重量，DCW），而野生型菌株约为1114 μg g⁻¹（DCW），提高了125.5%。此外，对突变体E5042生产虾青素的发酵条件进行了优化。在50 L发酵罐中对优化条件下的虾青素产量进行了放大研究。
利用低能离子束注入获得了一株虾青素高产且遗传稳定的突变菌株。该突变体可能是虾青素工业化生产的合适候选菌株。
低能离子束注入可有效提高红发夫酵母中虾青素的产量，这是微生物突变育种中的一项新技术。本研究获得的突变体有望用于虾青素的工业化生产。
