Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Laboratory of Cellular Biotechnology, CH-1015 Lausanne, Switzerland.
J Biotechnol. 2010 Jul 20;148(2-3):128-32. doi: 10.1016/j.jbiotec.2010.05.003. Epub 2010 May 25.
Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yields of up to 40 mg/L were achieved in simple batch cultures. The steady-state levels of the IgG light and heavy chain mRNAs were nearly 10 times higher than in the untreated control transfection even though the level of transfected plasmid DNA was the same in the presence or absence of VPA. The combination of VPA treatment and incubation of the transfected cells in mildly hypothermic conditions resulted in recombinant antibody yields of over 90 mg/L by 6 days post-transfection in batch cultures. The results demonstrated that the treatment of transfected CHO DG44 cells with VPA is a cost-effective strategy for enhancing transient gene expression by increasing the transgene mRNA levels.
丙戊酸（VPA）是一种小分子，可抑制组蛋白去乙酰化酶的活性。在这里，我们报告说 VPA 可增加瞬时转染的 CHO DG44 细胞中重组 mRNA 和蛋白的水平。在 VPA 的存在下，在简单的分批培养中，瞬时重组抗体的产量高达 40mg/L。尽管在有无 VPA 的情况下转染质粒 DNA 的水平相同，但 IgG 轻链和重链 mRNA 的稳态水平比未处理的对照转染高近 10 倍。VPA 处理与转染细胞在轻度低温条件下孵育的组合导致在分批培养中 6 天后的重组抗体产量超过 90mg/L。结果表明，用 VPA 处理转染的 CHO DG44 细胞是通过增加转基因 mRNA 水平来增强瞬时基因表达的一种具有成本效益的策略。
