Connor E A, Sugarman H, Rotshenker S
Department of Neurobiology, Stanford University Medical School, CA.
J Neurocytol. 1991 Apr;20(4):323-31. doi: 10.1007/BF01235549.
The anatomical distribution of a frog skeletal muscle antigen was studied using immunofluorescence microscopy and a monoclonal antibody 3B6 that was produced against denervated skeletal muscle. In innervated muscles, the monoclonal antibody 3B6 stain was associated with the inner surface of the muscle plasma membrane at the endplate and myotendinous junction. After denervation, the monoclonal antibody 3B6 stain extended from the endplate laterally around the perimeter of muscle fibres and longitudinally well beyond the endplate for a total length of 600-1000 microns. The monoclonal antibody 3B6 stain thus forms a cylindrical structure centred on the endplate. This observation shows that denervation produces a non-homogeneous molecular change in skeletal muscle fibres: an antigen that is present in high concentrations at innervated endplates appears in restricted perijunctional regions of denervated muscle fibres. It further suggests that perijunctional regions of denervated muscle fibres differ from the remaining non-endplate regions in molecular composition and possibly also in function.
利用免疫荧光显微镜和一种针对失神经支配骨骼肌产生的单克隆抗体3B6，研究了青蛙骨骼肌抗原的解剖分布。在有神经支配的肌肉中，单克隆抗体3B6染色与终板和肌腱连接处的肌质膜内表面相关。去神经支配后，单克隆抗体3B6染色从终板向肌纤维周边横向延伸，并纵向延伸至终板以外很远的地方，总长度为600 - 1000微米。因此，单克隆抗体3B6染色形成了一个以终板为中心的圆柱形结构。这一观察结果表明，去神经支配会在骨骼肌纤维中产生非均匀的分子变化：在有神经支配的终板中高浓度存在的一种抗原出现在失神经支配肌纤维的受限的结周区域。这进一步表明，失神经支配肌纤维的结周区域在分子组成上与其余非终板区域不同，在功能上可能也不同。
