用于人类胚胎干细胞长期生长的合成聚合物涂层。
Synthetic polymer coatings for long-term growth of human embryonic stem cells.
机构信息
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA.
出版信息
Nat Biotechnol. 2010 Jun;28(6):581-3. doi: 10.1038/nbt.1631. Epub 2010 May 30.
Abstract
We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.
摘要
我们报告了一种完全定义的合成聚合物涂层，聚[2-（甲基丙烯酰氧基）乙基二甲基-（3-磺丙基）铵氢氧化物]（PMEDSAH），它可以在几种不同的培养基中维持人类胚胎干细胞（hES）的长期生长，包括市售的定义明确的培养基。为 hES 细胞开发标准化、可控和可持续的培养基质是阐明控制 hES 细胞行为的机制以及优化 hES 细胞在生物医学应用中的条件的重要步骤。
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