Rodrigues Jhenifer Kliemchen, Dib Luciana Azôr, Ferriani Rui Alberto, Jordão Junior Alceu Afonso, Navarro Paula Andrea de Albuquerque Sales
Area de Concentração Biologia da Reprodução pelo Departamento de Ginecologia e Obstetrícia da Faculdade de Medicina de Ribeirão Preto - USP - Ribeirão Preto, SP, Brasil.
Rev Bras Ginecol Obstet. 2010 Mar;32(3):118-25. doi: 10.1590/s1516-84842010005000079.
To compare the serum levels of five markers of oxidative stress and assisted reproduction (AR) outcomes among infertile patients, with tubal and/or male factor and with polycystic ovary syndrome (PCOS).
70 patients were included, 58 with tubal and/or male factor infertility and 12 with PCOS, who underwent controlled ovarian stimulation to perform intracytoplasmic sperm injection (ICSI). A blood sample was collected between the third and fifth day of the menstrual cycle in the month prior to ovarian stimulation. We analyzed the levels of malondialdehyde, hydroperoxides, protein oxidation products, glutathione and vitamin E, by reading the absorbance with a spectrophotometer and by high performance liquid chromatography (HPLC). Data were analyzed statistically by the Student's t-test and Fisher's exact test.
Significant increases in the body mass index, ovarian volume and number of antral follicles were observed in PCOS patients, as well as the use of a lower total dose of follicle stimulating hormone for these patients. There were no differences in the response to ovarian stimulation, in the results of AR or serum levels of malondialdehyde, hydroperoxides, advanced oxidation protein products, glutathione and vitamin E between groups.
The present data did not demonstrate a difference in the levels of serum markers of oxidative stress or in AR results when comparing non-obese infertile patients with PCOS and controls. These data suggest that the results of AR may not be compromised in this specific subgroup of patients with PCOS. However, interpretations of the action of oxidative stress on the results of AR are still not clear and the reproductive implications of oxidative stress need to be better evaluated.
比较输卵管和/或男性因素不孕患者以及多囊卵巢综合征（PCOS）不孕患者的氧化应激五项标志物血清水平和辅助生殖（AR）结局。
纳入70例患者，其中58例为输卵管和/或男性因素不孕患者，12例为PCOS患者，她们均接受了控制性卵巢刺激以进行卵胞浆内单精子注射（ICSI）。在卵巢刺激前一个月的月经周期第3至5天采集血样。我们通过用分光光度计读取吸光度以及高效液相色谱法（HPLC）分析丙二醛、氢过氧化物、蛋白质氧化产物、谷胱甘肽和维生素E的水平。数据采用学生t检验和Fisher精确检验进行统计学分析。
观察到PCOS患者的体重指数、卵巢体积和窦卵泡数量显著增加，并且这些患者使用的促卵泡激素总剂量较低。两组之间在卵巢刺激反应、AR结果或丙二醛、氢过氧化物、晚期氧化蛋白产物、谷胱甘肽和维生素E的血清水平方面没有差异。
在比较非肥胖的PCOS不孕患者和对照组时，目前的数据未显示氧化应激血清标志物水平或AR结果存在差异。这些数据表明，在这一特定的PCOS患者亚组中，AR结果可能未受影响。然而，氧化应激对AR结果的作用仍不清楚，氧化应激对生殖的影响需要更好地评估。
