Department of Pharmaceutical Engineering, Sangji University, Wonju, Korea.
Arch Pharm Res. 2010 May;33(5):703-8. doi: 10.1007/s12272-010-0509-6. Epub 2010 May 29.
Young and mature Artemisia princeps var. orientalis (APO, Compositae) are used as a health food and a medicinal plant, respectively, in Korea. Here, we identified the in vitro potent peroxynitrite (ONOO(-))-scavenging effect (IC(50), 0.26 microg/mL) of the components from the EtOAc fraction. Octadecylsilane column chromatography on the EtOAc fraction yielded two caffeoylquinic acid compounds, 3,5-di-O-caffeoyl-muco-quinic acid (1) and methyl 4,5-di-O-caffeoylquinate (2) by NMR spectroscopic data, which have not been reported before from APO. The IC(50) values of compounds 1 and 2 were 0.18 +/- 0.01 microg/mL and 0.12 +/- 0.00 microg/mL, respectively, lower than that of the positive control (L-penicillamine). HPLC data indicated that young APO (1: 30.3 mg/g dried weight, 2: 27.7 mg/g) contained considerably higher quantities of the two caffeoylquinic acids than mature APO (1: 1.77 mg/g dried weight, 2: 4.10 mg/g).
在韩国，年轻和成熟的黄花蒿（APO，菊科）分别被用作保健食品和药用植物。在这里，我们鉴定出来自乙酸乙酯部分的成分具有体外高效的过氧亚硝酸盐（ONOO（-））清除作用（IC（50），0.26μg/mL）。十八烷基硅烷柱色谱法对乙酸乙酯部分进行分离，得到两种咖啡酰奎宁酸化合物，3,5-二-O-咖啡酰- muco-奎宁酸（1）和甲基 4,5-二-O-咖啡酰奎宁酸（2），通过 NMR 波谱数据，这两种化合物以前从未在 APO 中报道过。化合物 1 和 2 的 IC（50）值分别为 0.18 +/- 0.01μg/mL 和 0.12 +/- 0.00μg/mL，低于阳性对照（L-青霉胺）。HPLC 数据表明，年轻的 APO（1：30.3mg/g 干重，2：27.7mg/g）中两种咖啡酰奎宁酸的含量明显高于成熟的 APO（1：1.77mg/g 干重，2：4.10mg/g）。
