Department of Zoology/Zoophysiology, University of Gothenburg, Gothenburg, Sweden.
Cell Tissue Res. 2010 Jul;341(1):73-81. doi: 10.1007/s00441-010-0979-3. Epub 2010 May 30.
Glial fibrillary acidic protein (GFAP) is a commonly used marker to identify enteric glia in the mammalian gut. Little is however known about enteric glia in other vertebrates. The aim of the present study was to examine the distribution of GFAP immunoreactivity in adult and developing fish. In adult shorthorn sculpin (Myoxocephalus scorpius) and zebrafish (Danio rerio), GFAP immunoreactivity was seen in the myenteric plexus in all regions of the gut. Co-staining for the neuronal markers Hu C/D and acetylated tubulin showed that GFAP immunoreactivity was not associated with nerves. GFAP immunoreactivity was predominantly seen in processes with few glial cell bodies being demonstrated in adult fish. GFAP immunoreactivity was also found in the gut in larval zebrafish from 3 days post-fertilisation, i.e. at approximately the same time that differentiated enteric nerve cells first occur. Immunoreactivity was most prominent in areas with no or a low density of Hu-immunoreactive nerve cell bodies, indicating that the developing glia follows a different pattern from that of enteric neurons. The results suggest that GFAP can be used as a marker for enteric glia in fish, as in birds and mammals. The distribution of GFAP immunoreactivity implies that enteric glia are widespread in the fish gastrointestinal tract. Glia and neurons diverge early during development of the gastrointestinal tract.
胶质纤维酸性蛋白（GFAP）是一种常用于鉴定哺乳动物肠道内肠胶质细胞的标志物。然而，关于其他脊椎动物的肠胶质细胞却知之甚少。本研究旨在研究成年和发育中的鱼类中 GFAP 免疫反应性的分布。在成年短角石首鱼（Myoxocephalus scorpius）和斑马鱼（Danio rerio）中，GFAP 免疫反应性可见于整个肠道的肌间神经丛中。与神经元标志物 Hu C/D 和乙酰化微管蛋白的共染色表明，GFAP 免疫反应性与神经无关。GFAP 免疫反应性主要见于少突胶质细胞体的过程中，在成年鱼中仅可见少数胶质细胞体。在受精后 3 天的幼鱼斑马鱼的肠道中也发现了 GFAP 免疫反应性，即在分化的肠神经细胞首次出现的大致相同的时间。在 Hu 免疫反应性神经细胞体密度低或无的区域，免疫反应性最为明显，表明发育中的胶质细胞遵循与肠神经元不同的模式。研究结果表明，GFAP 可以像在鸟类和哺乳动物中一样，作为鱼类肠胶质细胞的标志物。GFAP 免疫反应性的分布表明肠胶质细胞在鱼类胃肠道中广泛存在。胶质细胞和神经元在胃肠道发育的早期就开始分化。
