Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
J Sep Sci. 2010 Jul;33(13):1904-8. doi: 10.1002/jssc.201000136.
A simple, specific and sensitive HPLC method has been developed for the determination of metoprolol in human plasma and urine. Separation of metoprolol and atenolol (internal standard) was achieved on an Ace C(18) column (5 microm, 250 mm x 4.6 mm id) using fluorescence detection with lambda(ex)=276 nm and lambda(em)=296 nm. The mobile phase consists of methanol-water (50:50, v/v) containing 0.1% TFA. The analysis was performed in less than 10 min with a flow rate of 1 mL/min. The assay was linear over the concentration range of 3-200 and 5-300 ng/mL for plasma and urine, respectively. The LOD were 1.0 and 1.5 ng/mL for plasma and urine, respectively. The LOQ were 3.0 and 5.0 ng/mL for plasma and urine, respectively. The extraction recoveries were found to be 95.6 +/- 1.53 and 96.4 +/- 1.75% for plasma and urine, respectively. Also, the method was successfully applied to three patients with hypertension who had been given an oral tablet of 100 mg metoprolol.
已经开发出一种简单、特异和灵敏的 HPLC 方法，用于测定人血浆和尿液中的美托洛尔。美托洛尔和阿替洛尔（内标）在 Ace C(18)柱（5 µm，250 mm x 4.6 mm id）上分离，荧光检测波长为 lambda(ex)=276 nm 和 lambda(em)=296 nm。流动相由甲醇-水（50:50，v/v）组成，含有 0.1% TFA。分析在 10 分钟内完成，流速为 1 mL/min。该测定法在血浆和尿液中的浓度范围分别为 3-200 和 5-300 ng/mL 时呈线性。血浆和尿液的 LOD 分别为 1.0 和 1.5 ng/mL，LOQ 分别为 3.0 和 5.0 ng/mL。血浆和尿液的提取回收率分别为 95.6 +/- 1.53%和 96.4 +/- 1.75%。此外，该方法已成功应用于 3 名高血压患者，他们口服了 100 mg 美托洛尔片。
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