Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240 Erzurum, Turkey.
Talanta. 2009 Nov 15;80(1):346-51. doi: 10.1016/j.talanta.2009.06.079. Epub 2009 Jul 8.
A sensitive and efficient method was developed for determination of metoprolol in human plasma by gas chromatography-mass spectrometry (GC-MS). Metoprolol and atenolol (internal standard, IS) were extracted from human plasma with a mixture of ethylacetate and diethylether at basic pH with liquid-liquid extraction. Calibration curves were linear over the concentration range 15-500 ng/ml. Intra- and inter-day precision values for metoprolol in human plasma were less than 6.4, and accuracy (relative error) was better than 8.8%. The analytical recovery of metoprolol from human plasma averaged 91.20%. The limits of detection (LOD) and quantification (LOQ) of metoprolol were 5.0 and 15 ng/ml, respectively. Also the developed and validated GC-MS method was successfully applied to three patients with hypertension who had been given an oral tablet of 100mg metoprolol.
建立了一种灵敏、高效的测定人血浆中美托洛尔的气相色谱-质谱（GC-MS）法。美托洛尔和阿替洛尔（内标，IS）在碱性 pH 条件下用乙酸乙酯和乙醚的混合物从人血浆中提取，采用液-液萃取法。美托洛尔在 15-500ng/ml 浓度范围内呈线性。人血浆中美托洛尔的日内和日间精密度值均小于 6.4%，准确度（相对误差）优于 8.8%。美托洛尔从人血浆中的平均分析回收率为 91.20%。美托洛尔的检测限（LOD）和定量限（LOQ）分别为 5.0 和 15ng/ml。所建立和验证的 GC-MS 方法成功应用于 3 例高血压患者，这些患者口服了 100mg 美托洛尔片。
