孟鲁司特可保护大鼠睾丸免受缺血/再灌注损伤。
Montelukast protects against testes ischemia/reperfusion injury in rats.
作者信息
Ozturk Hulya, Ozturk Hayrettin, Gideroglu Kaan, Terzi Hakan, Bugdayci Guler
机构信息
Associate Professor, Duzce University Medical School, Department of Pediatric Surgery, Duzce, Turkey;
出版信息
Can Urol Assoc J. 2010 Jun;4(3):174-9. doi: 10.5489/cuaj.09072.
INTRODUCTION
In this study, we investigate the effect of montelukast on histologic damage induced by testicular torsion-detorsion in rats.
METHODS
Twenty-one male Sprague-Dawley rats were separated into 3 groups, each containing 7 rats. A sham operation was performed in group 1 (control). In group 2 (ischemia-reperfusion [IR]/untreated), 1-hour detorsion of the testis was performed after 6 hours of unilateral testicular torsion. In group 3 (I-R/dextroamphetamine), after performing the same surgical procedures as in group 2, montelukast was given intraperitoneally. In all experimental rats, ipsilateral orchiectomies were performed for histological examination and tissue malondialdehyde (MDA), glutathione and myeloperoxidase assays.
RESULTS
Montelukast treatment significantly decreased the I-R-induced elevation in testes tissue MDA and glutathione levels were found to be preserved. The level of myeloperoxidase (MPO) activity was significantly increased in the testes tissue of the IR/untreated group. However, in I-R/montelukast treatment group significantly decreased testes tissue MPO level. Histopathologically, the in the group 2 rats, edema, congestion, hemorrhage between seminiferous tubules and necrosis of the germinal cells were predominant features in sections. However, most of the specimens in the montelukast treated group 3 showed grades-I and II injury. Additionally, the testicular injury score was lower in group 3 rats compared with group 2.
CONCLUSION
The current findings demonstrate that the montelukast decreased the severity of testicular injury by reversing the oxidative effects of testes I-R.
引言
在本研究中，我们调查了孟鲁司特对大鼠睾丸扭转-复位所致组织学损伤的影响。
方法
将21只雄性Sprague-Dawley大鼠分为3组，每组7只。第1组（对照组）进行假手术。第2组（缺血-再灌注[IR]/未治疗组），在单侧睾丸扭转6小时后进行1小时的睾丸复位。第3组（I-R/右旋苯丙胺组），在进行与第2组相同的手术操作后，腹腔注射孟鲁司特。对所有实验大鼠进行同侧睾丸切除术，以进行组织学检查以及组织丙二醛（MDA）、谷胱甘肽和髓过氧化物酶检测。
结果
孟鲁司特治疗显著降低了缺血-再灌注诱导的睾丸组织MDA升高，且谷胱甘肽水平得以维持。未治疗的缺血-再灌注组睾丸组织中髓过氧化物酶（MPO）活性水平显著升高。然而，在缺血-再灌注/孟鲁司特治疗组中，睾丸组织MPO水平显著降低。组织病理学上，第2组大鼠切片中的主要特征为水肿、充血、生精小管间出血以及生殖细胞坏死。然而，孟鲁司特治疗的第3组中的大多数标本显示为I级和II级损伤。此外，第3组大鼠的睾丸损伤评分低于第2组。
结论
当前研究结果表明，孟鲁司特通过逆转睾丸缺血-再灌注的氧化作用降低了睾丸损伤的严重程度。
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