Department of Obstetrics and Gynecology, Wakayama Medical University, 811-1 Kimi-idera, Wakayama 641-0012, Japan.
Int J Oncol. 2010 Jul;37(1):125-31. doi: 10.3892/ijo_00000660.
Patients with unresectable advanced uterine leiomyosarcoma have a very poor prognosis because no effective chemotherapeutic protocols exist. There are currently few established primary human uterine leiomyosarcoma cell lines that can be used to investigate effective therapies. To overcome this problem, we carried out long-term in vitro cell culture and/or nude mouse transplantation and successfully established novel human uterine leiomyosarcoma cell lines from extrauterine and intrauterine tumors that were surgically excised from a single patient. The established cells were characterized by flow cytometry, anticancer drug-sensitivity assays and karyotyping analyses. All the established cell lines showed unstable multiple chromosome abnormalities. Since the cells can proliferate in vitro and in vivo, they will be useful for developing new therapeutic strategies for advanced uterine leiomyosarcoma patients.
对于无法切除的晚期子宫平滑肌肉瘤患者，由于目前尚无有效的化疗方案，预后极差。目前，能够用于研究有效治疗方法的已建立的原发性人子宫平滑肌肉瘤细胞系很少。为了克服这个问题，我们进行了长期的体外细胞培养和/或裸鼠移植，并成功地从单个患者手术切除的子宫外和子宫内肿瘤中建立了新型的人子宫平滑肌肉瘤细胞系。通过流式细胞术、抗癌药物敏感性测定和染色体核型分析对建立的细胞系进行了特征描述。所有建立的细胞系均显示出不稳定的多发性染色体异常。由于这些细胞可以在体外和体内增殖，它们将有助于为晚期子宫平滑肌肉瘤患者开发新的治疗策略。
