The binding of corticosterone to the class-theta glutathione S-transferase from the eyes of the shrimp Penaeus japonicus (Crustacea: Decapoda).
作者信息
Shu-Fen T, Chuang N N
机构信息
Division of Biochemistry and Molecular Science, Institute of Zoology, Academia Sinica,Nankang, Taipei, Taiwan, Republic of China.
出版信息
Comp Biochem Physiol Biochem Mol Biol. 1994 Jun;108(2):215-9.
PMID:20514730
Abstract
The anionic homodimeric theta glutathione S-transferase (EC 2.5.1.18), with specific activities of 4.6 units/mg with 1-chloro-2,4-dinitrobenzene as substrate, from the eyes of the shrimp Penaeus japonicus, binds to corticosterone, which was confirmed by the analysis of the conjugate by SDS-PAGE and fluorography of proteins with bound (3)H-corticosterone.The reaction of the theta glutathione S-transferase with corticosterone was prevented by the presence of glutathione at 10 mM.
摘要
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