Kameda Keiko, Ikawa Kazuro, Ikeda Kayo, Morikawa Norifumi, Nakashima Akira, Ohge Hiroki, Sueda Taijiro
Department of Clinical Pharmacotherapy, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan.
J Chromatogr Sci. 2010 May-Jun;48(5):406-11. doi: 10.1093/chromsci/48.5.406.
A high-performance liquid chromatography (HPLC) method using ultrafiltration to pretreat peritoneal fluid and bile samples is developed to measure meropenem and biapenem concentrations in human peritoneal fluid and bile. Meropenem or biapenem in peritoneal fluid or bile samples is stabilized by mixing with 1 mol/L 3-morpholinopropanesulfonic acid buffer (pH 7.0) (1:1). The mixture is transferred to a Nanosep 10K centrifugal filter device; after centrifugation, the filtrate is subjected to reversed-phase HPLC, and the eluate is monitored at 300 nm. No interference from endogenous substances is observed. The lower limits of quantification are 0.05 microg/mL for peritoneal fluid and 0.1 microg/mL for bile. The new method has been applied to comparative site-specific-pharmacokinetic investigations in surgery patients.
开发了一种利用超滤预处理腹膜液和胆汁样本的高效液相色谱（HPLC）方法，用于测定人腹膜液和胆汁中美罗培南和比阿培南的浓度。腹膜液或胆汁样本中的美罗培南或比阿培南通过与1 mol/L 3-吗啉丙磺酸缓冲液（pH 7.0）（1:1）混合来稳定。将混合物转移至Nanosep 10K离心过滤装置；离心后，将滤液进行反相HPLC分析，并在300 nm处监测洗脱液。未观察到内源性物质的干扰。腹膜液的定量下限为0.05 μg/mL，胆汁的定量下限为0.1 μg/mL。该新方法已应用于手术患者的比较性位点特异性药代动力学研究。
