一种改进的传染性朊病毒细胞间传播方法。
An improved method for cell-to-cell transmission of infectious prion.
作者信息
Tanaka Masahiko, Hara Hideyuki, Nishina Hiroshi, Hanada Kentaro, Hagiwara Ken'ichi, Maehama Tomohiko
机构信息
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
出版信息
Biochem Biophys Res Commun. 2010 Jul 2;397(3):505-8. doi: 10.1016/j.bbrc.2010.05.143. Epub 2010 May 31.
Prion diseases are characterized by the accumulation of a pathological form of prion protein (PrP(Sc)), which behaves as an infectious agent. Here we developed an in vitro co-culture system to analyze the PrP(Sc) transmission from ScN2a cell, which persistently retains PrP(Sc), to naïve N2a cell. In this cell-to-cell transmission system, PrP(Sc) transmitted to recipient N2a cell was able to be detected within 5-7days. Further characterization showed that higher cell density greatly facilitated the transmission of PrP(Sc). This improved in vitro transmission method may become a useful tool for unveiling the molecular mechanism of PrP(Sc) transmission.
朊病毒疾病的特征是一种病理性朊病毒蛋白（PrP(Sc)）的积累，该蛋白具有传染性。在此，我们开发了一种体外共培养系统，以分析PrP(Sc)从持续保留PrP(Sc)的ScN2a细胞向未感染的N2a细胞的传播。在这个细胞间传播系统中，5 - 7天内就能检测到传递给受体N2a细胞的PrP(Sc)。进一步的特征分析表明，较高的细胞密度极大地促进了PrP(Sc)的传播。这种改进的体外传播方法可能成为揭示PrP(Sc)传播分子机制的有用工具。
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