Bando A, Orita T, Matsumoto K, Yoneyama A, Kitamura K, Nakahara K, Ohkubo A
Department of Laboratory Medicine, Tokyo University Medical School.
Rinsho Byori. 1991 Mar;39(3):278-82.
The lymphocyte surface marker analysis with flow-cytometry is usually performed using a density gradient centrifugal method or a whole blood method. We have compared these two methods using several monoclonal antibodies and have found the significant difference in positivity all monoclonal antibodies except TCR-1 (anti alpha/beta receptor on T-lymphocyte). We found the significantly higher positivity of Leu12 and HLA-DR as well as lower of the other monoclonal antibodies by using whole blood method than by density gradient centrifugal method. We conclude that it is necessary to make a distinct description of the method used in the surface marker analysis.
流式细胞术进行淋巴细胞表面标志物分析通常采用密度梯度离心法或全血法。我们使用几种单克隆抗体对这两种方法进行了比较，发现除TCR-1（抗T淋巴细胞上的α/β受体）外，所有单克隆抗体的阳性率存在显著差异。我们发现，与密度梯度离心法相比，全血法检测时Leu12和HLA-DR的阳性率显著更高，而其他单克隆抗体的阳性率更低。我们得出结论，有必要对表面标志物分析中使用的方法进行明确描述。
