Kobayashi M, Takaya S, Koie H, Nagai K
Department of Surgery, Hirosaki University School of Medicine, Aomori, Japan.
Jpn J Surg. 1991 Mar;21(2):210-15. doi: 10.1007/BF02470910.
This study focused on the glomerular structural changes observed after cyclosporine A (CsA)-induced nephrotoxicity. Renal structural changes were examined in rats treated with oral CsA, given as a daily dose of 50 mg/kg for periods of up to 49 days. By means of scanning electron microscopy and morphometry, we first demonstrated that the most conspicuous and reproducible ultrastructural changes could be detected in the endothelial cells of the glomerular capillaries. These changes included a reduction in the fenestral pore size and partial disappearance of endothelial fenestration, the appearance of microvilli-like projections on the endothelial surface, and flattening and widening of the cytoplasmic folds. We believe that the ultrastructural changes observed in this study are partially responsible for the alterations in renal function seen in the cyclosporine A-treated model, and that these alterations are caused by CsA-induced vasospasm.
本研究聚焦于环孢素A（CsA）诱导的肾毒性后所观察到的肾小球结构变化。对口服CsA（每日剂量50mg/kg，给药长达49天）的大鼠进行肾脏结构变化检查。通过扫描电子显微镜和形态测量学，我们首先证明，在肾小球毛细血管内皮细胞中可检测到最明显且可重复的超微结构变化。这些变化包括窗孔孔径减小和内皮窗孔部分消失、内皮表面出现微绒毛样突起以及细胞质褶皱变平变宽。我们认为，本研究中观察到的超微结构变化部分导致了环孢素A治疗模型中所见的肾功能改变，且这些改变是由CsA诱导的血管痉挛引起的。
