日本脑炎大鼠模型血清细胞因子和趋化因子的序贯变化。
Sequential changes in serum cytokines and chemokines in a rat model of Japanese encephalitis.
机构信息
Department of Neurology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
出版信息
Neuroimmunomodulation. 2010;17(6):411-6. doi: 10.1159/000291438. Epub 2010 May 27.
OBJECTIVES
The mechanisms underlying inflammation and immune responses in viral encephalitis are not fully understood. Therefore, in the present study we aimed to investigate the cytokine and chemokine levels in Japanese encephalitis virus (JEV)-infected rats.
METHODS
Twelve-day-old Wistar rats were infected with 3 x 10(6) plaque-forming units of JEV intracerebrally. Cytokine and chemokine levels were analyzed in serum 3, 6, 10 and 20 days post inoculation (dpi).
RESULTS
There were increased levels of proinflammatory and anti-inflammatory cytokines and a chemokine (monocyte chemoattractant protein-1) in the serum of rats after JEV infection compared to controls. The levels of cytokines and chemokine peaked at 10 dpi and had declined significantly by 20 dpi. The neurological deficit also increased in the acute stage of disease and partially recovered thereafter.
CONCLUSION
Serum cytokine and chemokine levels decline at 10 dpi and do not significantly correlate with neurological dysfunction.
目的
病毒脑炎中炎症和免疫反应的机制尚不完全清楚。因此，本研究旨在探讨日本脑炎病毒（JEV）感染大鼠的细胞因子和趋化因子水平。
方法
将 12 日龄 Wistar 大鼠用 3 x 10(6)噬菌斑形成单位的 JEV 经脑内感染。在接种后 3、6、10 和 20 天分析血清中的细胞因子和趋化因子水平。
结果
与对照组相比，JEV 感染后的大鼠血清中促炎和抗炎细胞因子以及趋化因子（单核细胞趋化蛋白-1）的水平升高。细胞因子和趋化因子水平在 10 dpi 时达到峰值，并在 20 dpi 时显著下降。神经功能缺损在疾病的急性期增加，并在随后部分恢复。
结论
血清细胞因子和趋化因子水平在 10 dpi 时下降，与神经功能障碍无明显相关性。
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