Sluvko A L, Bel'chenko D I
Antibiotiki. 1978 Mar;23(3):247-9.
Peculiar characteristics of the antigenic properties of penicillin compounds with heterological and homological beta-lipoproteids of the blood serum were investigated. The beta-lipoproteids isolated from the blood serum of humans (donors) and rabbits were mixed with potassium benzylpenicillin (10000 and 50000 gamma/ml). Twenty seven rabbits were immunized with such compounds and solutions of beta-lipoproteids without addition of the antibiotic. The antisera obtained from the animals were used in the reaction of precipitation and immunoelectrophoresis, the results of which were densitometrized. Some physico-chemical properties of the preparations, such as pH, electrokinetic features were also studied. It was shown that the immunochemical properties of heterological beta-lipoproteids, i.e. precipitation bands changed due to the effect of penicillin. They differed from the control by compactness and contrastness, their amounts decreased, a separate band not identical to that of the control antigen appeared. A separate or intermediate antigen was found in the experiments with serum exhaustion. The peculiarities of the precipitation reaction were confirmed by the immunoelectrophoregrams. These differences were also registered on the densitograms. The compounds of penicillin with homological beta-lipoproteids of the rabbit blood serum induced formation of specific precipitins in the animals. Some physico-chemical properties of beta-lipoproteids also changed under the effect of penicillin.
研究了青霉素与血清中异源和同源β-脂蛋白化合物抗原特性的特殊性质。从人（供体）和兔血清中分离出的β-脂蛋白与苄青霉素钾（10000和50000γ/ml）混合。用这些化合物和未添加抗生素的β-脂蛋白溶液对27只兔进行免疫。从动物获得的抗血清用于沉淀反应和免疫电泳，其结果进行光密度测定。还研究了制剂的一些物理化学性质，如pH值、电动特性。结果表明，异源β-脂蛋白的免疫化学性质，即沉淀带因青霉素的作用而发生变化。它们在致密性和对比度方面与对照不同，其数量减少，出现了一条与对照抗原不同的单独条带。在血清耗尽实验中发现了单独的或中间的抗原。免疫电泳图谱证实了沉淀反应的特殊性。这些差异也记录在密度图上。青霉素与兔血清同源β-脂蛋白的化合物在动物体内诱导形成特异性沉淀素。β-脂蛋白的一些物理化学性质在青霉素的作用下也发生了变化。
